The group of Siegfried Musser from Texas AM University recently published an article in Nature Cell Biology where they used a MIRAO 52E deformable mirror to perform nuclear pore complex imaging using adaptive optics in super resolution. Super resolution...
Watch or rewatch the presentation given by Antoine Hubert (Imagine Optic and ESPCI) at the European Conference on Biomedical Optics (ECBO) on June 21st. Antoine presents his latest results on an Extended-Scene Shack-Hartmann wavefront sensing-based adaptive optics...
Correction of static aberrations involved in tilted objective geometries restores the performance and full versatility of open-top light-sheet configurations. Even if many optical geometries have been proposed in light-sheet microscopy, in particular regarding the...
The complexity of the aberration detection and correction process for PALM/STORM microscopy in biological samples has long been a limiting factor for widespread use of adaptive optics in biological imaging. The closed-loop...
A MicAO 3DSR adaptive optics system has recently been installed in the laboratory of Pablo Rivera Fuentes at École Polytechnique Fédérale de Lausanne (EPFL). Pablo chose the system because it provides the best axial localization precision due to astigmatic PSF induced...
GCaMP7 labelled neurons of the live, adult drosophila brain at 45µm depth, imaged with fast, closed-loop adaptive optics (AO) on a Light-Sheet microscope, using our new direct wavefront sensing for microscopy approach - not requiring any guide star. All this has been...
Photoactivation localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM) enable localization of fluorescent molecules with nanometric resolution. In these super resolution microscopy methods the positions of molecules are obtained by...
Watch or rewatch "Adaptive Optics in SMLM for deep tissue imaging", our 2nd Webinar on Microscopy. Marijn Siemons from Utrecht University presents: - An introduction to SMLM and characterization of MICAO 3DSR device, - Depth-induced ellipticity loss in 3D SMLM - And...
Newly funded MaxPhase project will enable high-resolution, multiplexed phase sensing for metrology, laser characterization and non-invasive cell imaging. Phase measurement has been well established for decades as a valuable method in optical metrology and adaptive...
The 2020 edition of BIPSA conference (Excellence Network in Bioimaging and Health in Nouvelle Aquitaine) will be held November 9-10th as part of the ViV HEALTECH forum. This full day of conference will be dedicated to biological imaging in the morning and to medical...
In the past six years, adaptive optics (AO) has contributed to 8 Nobel Prizes. Unseen but essential, adaptive optics allows significant improvements in optical performance for imaging and laser systems... and Nobels. The 2014 chemistry laureates, Stefan Hell, Eric...
Watch or rewatch "PSF optimization for 3D SMLM super-resolution imaging using adaptive optics" the first webinar of our series on adaptive optics for microscopy. Fabrice Harms, Scientific Coordinator, and Audrius Jasaitis, Application Specialist, addressed fundamental...