Tripple the fluorescence signal
In multi photon microscopy, adaptive optics is implemented on the excitation pathway and can significantly boost the fluorescence signal from the biological sample. Correction of the system and sample-induced aberrations improves the focusing ability of the microscope, with a typical 3 to 5 times gain factor of the two-photon effect. The increase of fluorescence signal allows to reach much deeper layers of the sample or to decrease the intensity of the excitation laser in order to prevent the bleaching of the sample and minimize photo-toxicity effects.
Figure caption: Drosophila larva imaging by Third Harmonic Generation microscopy (THG) without (on the left) and with (on the right) adaptive optics. Correction of aberrations in this case boosts the fluorescence signal by a factor of 3 and new features inside the sample become visible (see zooms). Image produced in collaboration with Emmanuel Beaurepaire, LOB, Ecole Polytechnique, France.