Build Your Ideal Adaptive Optics System for Microscopy

AOKit Bio allows you to build the adaptive optics system that best suits your individual needs. Whichever microscopy method you are using, adaptive optics allows you to pre-compensate aberrations present in your imaging system and to improve your image quality.

AOKit Bio is a set of adaptive optics components, composed of a Shack-Hartmann wavefront sensor (HASO4 First for visible or HASO4 SWIR for the infrared spectral range), Mirao 52e or any other deformable mirror or phase modulator (please contact Imagine Optic in advance to check the compatibility and availability), as well as adaptive optics software. You can choose between the WaveKit Bio set of functions (SDK) or a fully functional adaptive optics software with a user interface. Both software control all functionalities of the wavefront sensor and deformable mirror, can operate these devices in closed and open-loop modes and contain image-based (sensorless) aberration detection algorithms (3N and phase diversity) adapted for microscopy applications.

We recommend implementing AOKit Bio for the following microscopy techniques: wide field, confocal, multiphoton, light sheet, STED and SIM microscopy, as well as FCS or optical tweezers.



The AOKit Bio consists of the HASO4 First wavefront sensor and the Mirao 52e electromagnetically deformable mirror. Here are specifications of Mirao 52e :

Description Specification
Number of actuators 52
Maximum generated wavefront (PV) ±50 µm
Surface quality (rms active flat) 10 nm
Integrated tip/tilt correction yes
Spatial frequency correction Zernike orders  up to 6
Effective diameter 15 mm
Linearity  >95%
Hysteresis <2%
Actuator input voltage  ±1V maximum*
Coating protected silver
Power consumption 50W max
Dimensions / weight 64 x 64 x 23 mm3 / 490g**
Connectivity USB 2.0
Operating System Windows XP, Windows 7 (32 and 64 bit) and Windows 10
(*) Total sum of applied voltages not to exceed 25V.

(**) Mirror unit only.

HASO4 First
Aperture dimension 3.6 x 4.6 mm²
Number of microlenses 32 x 40
Tilt dynamic range > ± 3 ° (400 λ)
Focus dynamic range ± 0.018 m to ± ∞ (350 λ)
Focus dynamic range – maximum NA 0.1
Repeatability (rms) < λ/200
Wavefront measurement accuracy in relative mode (rms)* ~ λ/150
Wavefront measurement accuracy in absolute mode (rms)** ~ λ/100
Tilt measurement sensitivity (rms) 5 μrad
Focus measurement sensitivity (rms) 3·10-3m-1
Spatial resolution ~ 110 μm
Maximum acquisition frequency 100 Hz
Processing frequency (CPU 3Ghz, 512 Mb RAM) 20 Hz
Working wavelength range 350 – 1100 nm
Calibrated wavelength in the 400-1100 nm range, details on request***
Extended wavelength range not available
Dimensions / weight 42x 42 x 42 mm3 /150g
Working temperature 15 – 30° C
Interface / Power supply USB 3.0 / 2.7 W via USB
Operating system Windows 7 (32 and 64 bit) and Windows 10
WaveViewTM metrology software included
External trigger yes
(*) Wavefront as seen by the device.

(**) Difference between the real wavefront and a reference wavefront obtained in similar conditions (5λ of shift max).

(***) Single wavelength calibration (+- 50 nm), second wavelength calibration available on request.


Service Documents

HASO4 First | Specification sheet

AOKit Bio

AOKit Bio | Specification sheet


mirao52e | Specification sheet

Publication list | Micao and AOkit Bio