Build Your Ideal Adaptive Optics System for Microscopy

The AOKit – bio allows you to build the adaptive optics system best suited to you individual needs. Whatever configuration you choose, it allows you to pre-compensate for your imaging system’s aberrations to start off with the best possible native quality. We equally offer a software development kit that allows you to build your own adaptive-optics software to perfectly meet your needs.

For custom-built setups and custom implementations of deformable mirror, Imagine Optic offers a package of key elements of adaptive optics, called AOKit Bio. This set includes the Shack-Hartmann type Haso4 First wavefront sensor and mirao 52e deformable mirror. These wavefront sensor and deformable mirror proved to be ideal solution for microscopy, already implemented on a number of different microscopy setups all over the world. AOKit Bio also includes the software program, which controls all the functionality of the wavefront sensor and deformable mirror and also contains the aberration correction and image improvement iterative algorithms (genetic, 2N+1 and modified 3N). Under request we also provide the Software Development Kit (SDK) source code so these devices could be managed from the customer build control software. AOKit Bio is typically implemented in these microscopy methods: multiphoton microscopy, SPIM or light sheet microscopy, but it is also possible to improve imaging in SIM, FCS , optical tweezers etc.



The AOKit BIO consists of the HASO4 First wavefront sensor and the mirao 52-e electromagnetically deformable mirror. Here are specifications of mirao 52-e :

Description Specification
Number of actuators 52
Maximum generated wavefront (PV) ±50 µm
Surface quality (rms active flat) 10 nm
Integrated tip/tilt correction yes
Spatial frequency correction Zernike orders  up to 6
Effective diameter 15 mm
Linearity  >95%
Hysteresis <2%
Actuator input voltage  ±1V maximum1
Coating protected silver
Power consumption 50W max
Dimensions / weight 64 x 64 x 23 mm / 490g2
Connectivity USB 2.0
Operating System Windows XP, 7 (x32 – x64 under evaluation)
(1) Total sum of applied voltages not to exceed 25V.

(2) Mirror unit only.

HASO4 First
Aperture dimension 3.6 x 4.6 mm²
Number of microlenses 32 x 40
Tilt dynamic range > ± 3 ° (400 λ)
Focus dynamic range ± 0.018 m to ± ∞ (350 λ)
Focus dynamic range – maximum NA 0.1
Repeatability (rms) < λ/200
Wavefront measurement accuracy in relative mode (rms)1 ~ λ/150
Wavefront measurement accuracy in absolute mode (rms)² ~ λ/100
Tilt measurement sensitivity (rms) 5 μrad
Focus measurement sensitivity (rms) 3·10-3m-1
Spatial resolution ~ 110 μm
Maximum acquisition frequency 100 Hz
Processing frequency (CPU 3Ghz, 512 Mb RAM) 20 Hz
Working wavelength range 350 – 1100 nm
Calibrated wavelength in the 400-1100 nm range, details on request3
Extended wavelength range not available
Dimensions / weight 42x 42 x 42 mm /150g
Working temperature 15 – 30° C
Interface / Power supply USB 3.0 / 2.7 W via USB
Operating system Win 7 (x86 / x64)
HASOv3 metrology software included
External trigger yes
(1) Wavefront as seen by the device.

(2) Difference between the real wavefront and a reference wavefront obtained in similar conditions (5λ of shift max).

(3) Single wavelength calibration (+- 50 nm), second wavelength calibration available on request.


Service Documents

HASO4 First

HASO4 First | Specification sheet

AOKit Bio

AOKit Bio | Specification sheet


mirao52e | Specification sheet

Publication list | Micao and AOkit Bio