Improve localization precision and add the third dimension
Applications
- Photoactivation localization microscopy (PALM)
- Stochastic optical reconstruction microscopy (STORM)
- Single particle tracking (SPT)
MicAO 3DSR is an adaptive optics device specifically designed for single molecule localization microscopy (SMLM) methods such as photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM) and single particle tracking (SPT). The high optical transmission of MicAO 3DSR and its ability to correct aberrations enables the detection of more photons from the sample, which, in turn, increases localization precision (resolution) of SMLM methods. Moreover, MicAO 3DSR can introduce aberration-free astigmatism for the most precise 3D SMLM imaging. Excellent temporal stability of the deformable mirror in an open-loop mode allows long-term image sequence acquisitions which is required by the SMLM methods.
MicAO 3DSR device has been developed for an easy implementation on any inverted-frame microscope. The device is simply inserted between the microscope side port and the imaging camera, and functions as an image relay, still allowing for the use of additional modules such as dual color view devices and microscope port splitters.
MicAO 3DSR is delivered with user friendly software which contains all the necessary tools for detection and correction of aberrations in biological samples.
For the integration of Adaptive Optics on an inverted frame microscope, MicAO 3DSR is the perfect solution. For custom setups, Imagine Optic offers a set of separate Adaptive Optics components: AOKit Bio (Mirao 52e deformable mirror, HASO4 First Shack-Hartmann wavefront sensor and adaptive optics software), which facilitates the design and implementation of your own setup.
Specifications
MICAO 3DSR
| Description | MICAO 3DSR | MICAO IR |
|---|---|---|
| Microscope compatibility | Standard inverted frames, right or left port | Standard inverted frames, right or left port |
| Objective compatibility | Oil 60x, NA<1.49 and 100x NA>1.40 | Oil 60x, NA<1.49 and 100x NA>1.40 |
| Optical transmission | T>95% 475-675nm, T>90% 400-700 nm | T>87% 700-1100 nm |
| Operating wavelength range | 400-700 nm | 700-1100 nm |
| Lateral localization precision* | 8nm @ 1000 photons and 5nm @ 4000 photons | 8nm @ 1000 photons and 5nm @ 4000 photons |
| Axial localization precision | 16nm @ 1000 photons and 10nm @ 4000 photons | 16nm @ 1000 photons and 10nm @ 4000 photons |
| Working environment | 20-25°C, 20-80% RH non-condensing | 20-25°C, 20-80% RH non-condensing |
| Wavefront temporal stability | <10nm RMS for min. 12h in working environment conditions | <10nm RMS for min. 12h in working environment conditions |
| Dimensions / weight | 432 x 325 x 140mm3 / 9kg | 432 x 325 x 140mm3 / 9kg |
| Adaptive Optics Software | MicAO software v1.3 | MicAO software v1.3 |
* typical performance
Publications
Downloads
Service Documents
Application Notes
Service Documents
PALM/STORM | Application note : Describes how MicAO 3DSR improves localization precision in PALM/STORM
SMLM Imaging | Application note : Demonstrates how MicAO 3DSR can be used for SMLM imaging deep in the sample
PALM/STORM Imaging | Application note : An example of PALM/STORM imaging of centrosomal proteins