For live cell imaging
Scanning confocal and spinning disk confocal microscopy are exceedingly popular imaging techniques in cellular biology for fixed cell and in vivo imaging. In these modalities, using adaptive optics allows the user to increase fluorescence signal and image contrast, especially when a high-magnification oil immersion objective is used to image water-based biological samples. Fixed biological samples, primarily imaged in scanning confocal microscopy, induce complex aberrations which distort the image, reduce resolution and sharpness. When imaging deeper in biological samples with the spinning disk technique, correction of aberrations using adaptive optics more than doubles the fluorescence signal. This also allows the PSF to be uniform in different parts of the sample, which dramatically improves the quality of image deconvolution when post processing the data.
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