Control your beam
Confocal microscopy is an interesting modality for fixed samples. Unfortunately the fixed sample induce complex aberrations which distort the image and reduce the resolution and sharpness of the image. The application of adaptive optics enables the user to correct those aberrations and to increase the fluorescence signal. When performing Z-stacks, our software allows depth-dependent correction of aberrations using the aberration model. The fluorescence signal and resolution can be enhanced for each layer of the sample. Adaptive optics also enables to have a uniform shape of the PSF in the whole sample which improves deconvoluted results.
The improvement of image quality with adaptive optics on fixed MDA-MB 231 cell at 35um depth in collagen type I 3D matrix fluorescently labeled for actin with phalloidine and Alexa Fluor 488. Aberrations were detected using 3N iterative algorithm (MicAO software) on all major Zernike modes. Courtesy of M. Irondelle (Chavrier team, Institut Curie, France).