For live imaging
The spinning disk confocal microscopy is exceedingly popular imaging method in cellular biology for in vivo imaging. The application of adaptive optics in this modality allows to increase the fluorescence signal, especially deep in the sample, when high magnification oil immersion objective is used to image water based samples. The MicAO software offers automatic correction of aberrations when performing Z-stacks, even without prior illumination of the sample. Using adaptive optics, the fluorescence signal can be more than doubled, especially deep in the sample. By using adaptive optics it is possible to obtain a uniform PSF in different parts of the sample. This dramatically improves the quality of image deconvolution.
Drosophila brain in vivo at 15µm depth. The correction of aberrations was performed using the linear model for spherical aberration. Courtesy Maria Rujano Maldonado (Basto team, Institut Curie, France).