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Optical Coherence Tomography? Nonlinear Microscopy (2-photon, SHG, THG, CARS)? Structured Light Illumination? Confocal Microscopy?
When it comes to adaptive optics for microscopy in life sciences, the images speak for themselves!
| Microscopy enhanced with adaptive optics is rapidly becoming a formidable ally for research in life sciences. Its unique ability to correct for specimen-induced aberrations has enabled scientists to acquire remarkably detailed images whereas once only vague perceptions were visible.
Recent years have seen the technology mature into a tool that can be harnessed by more and more professionals around the world. Only Imagine Optic, a leader in wavefront metrology and adaptive optics for over 12 years, provides a complete line of products that accompanies researchers in their quest to see better and to delve deeper into the organic.
The AOKit™ - bio is the one-stop solution that exceeds the performances of any product or solution available on the market today. The AOKit – bio combines a HASO™3 wavefront sensor adapted to your application, the acclaimed mirao™ 52-e Electromagnetic Deformable Mirror and CASAO™ command & control software for all-in-one loop control and instrument diagnostics. Even more, for those that want to reap the benefits of adaptive optics without a wavefront sensor, GENAO™ enables users to employ advanced genetic algorithms and merit-based selection criteria to achieve optimal image quality. |
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| Advantages |
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All-in-one
Patented and proprietary technologies direct from the original manufacturer
Unparalleled performance
mirao 52-e's ±50 µm stroke and exceptional optical quality, HASO3's impeccable accuracy and wide dynamic range, and CASAO or GENAO's unique software features allow you to quickly make the most out of your investment
Unrivaled experience
Imagine Optic has accompanied a host of researchers to get the most out of their AO systems
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Currently, our adaptive optics and wavefront metrology products are being used to improve the performance of various types of microscopy by your fellows at the Sedat Lab (USCF), Betzig Lab (Janelia Farm) , Holy Lab (University of Washington), So Lab (MIT), Department of Engineering Science (Oxford University), Laboratory for Optics and Biosciences (Ecole Polytechnique (LOB)), The Institute of Photonic Sciences (ICFO), and many others… To download an independant study on deformable mirror performance, click here.
| Third Harmonic Generation (THG) |
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Third Harmonic Generation (THG) Microscopy Drosophila larva - Images courtesy of Drs. Beaurepaire, Débarre & Olivier, Ecole Polytechnique, LOB (CNRS-INSERM), France.
The images below, approximately 25% of their original size, show a Drosophila larva imaged using Third Harmonic Generation microscopy. Left, uncorrected. Right, corrected with adaptive optics using mirao. The inset and the lower images are magnified versions of the white box region, showing increased contrast on small structures in the corrected image. Reference: "Dynamic aberration correction for multiharmonic microscopy", N. Olivier, D. Débarre et E. Beaurepaire, Opt. Lett. 34, 3145 (2009).
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| Second Harmonic Generation (SHG) |
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Second Harmonic Generation (SHG) Microscopy Drosophila larva - Images courtesy of Drs. Beaurepaire, Débarre & Olivier, Ecole Polytechnique, LOB (CNRS-INSERM), France.
The images below show,, approximately 35% of their original size, a Drosophila larva imaged using Second Harmonic Generation microscopy, simultaneously with the above THG images. Left, uncorrected. Right, corrected with adaptive optics using mirao. Notice the increase in signal and contrast. Reference: "Dynamic aberration correction for multiharmonic microscopy", N. Olivier, D. Débarre et E. Beaurepaire, Opt. Lett. 34, 3145 (2009). |
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| Third Harmonic Generation (THG) |
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Third Harmonic Generation (THG) Microscopy Plant tissue - Images courtesy of Drs. Beaurepaire, Débarre & Olivier, Ecole Polytechnique, LOB (CNRS-INSERM), France.
The images below, approximately 25% of their original size, show a fixed plant tissue sample imaged using Third Harmonic Generation microscopy. Left, uncorrected. Right, corrected with adaptive optics using mirao. |
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| 2-Photon-Excited Fluorescence (2PEF) |
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2-Photon-Excited fluorescence (2PEF) Microscopy Plant tissue - Images courtesy of Drs. Beaurepaire, Débarre & Olivier, Ecole Polytechnique, LOB (CNRS-INSERM), France.
The images below, approximately 35% of their original size, show a fixed plant tissue sample imaged using two-photon-excited fluorescence microscopy, simultaneously with the above THG images. Left, uncorrected. Right, corrected with adaptive optics using mirao. |
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| Intrinsic Third Harmonic Generation (THG) |
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Intrinsic third harmonic generation from a C. Elegans specimen - Images courtesy of Dr Martin Booth, EPSRC Advanced Research Fellow, Department of Engineering Science, University of Oxford.
The images below show intrinsic third harmonic generation from a C. Elegans specimen before and after aberration correction. They were acquired using a mode-locked Chromium-Forsterite laser at wavelength 1230nm, pulse length 65fs. The objective was 1.15NA water immersion, Olympus UApo/340, 40x. The focussing depth was 30um. The image width is 125um.
The aberrations include system aberrations (the illumination wavelength of 1230nm lies outside the specifications of the objective lens) and specimen-induced aberrations arising from focusing deep into the specimen. The aberrations were corrected using mirao in a sensorless adaptive optics scheme. |
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| 3D Wide-Field Fluorescence |
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Mouse cells - Images courtesy of Drs. Kner & Kam, UCSF Sedat Lab
The deconvolved images below, slightly enlarged and at 85% of quality with JPEG compression, of alexa488 stained actin in B16F10 mouse cells 4.5µm below the surface show samples imaged using 3D Wide-Field Fluorescence microscopy. Left, uncorrected. Right, corrected with adaptive optics using mirao. |
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200 nm beed at 67 µm below the cover slip - Images courtesy of Drs. Kner & Kam, UCSF Sedat Lab
Images 1 through 4 from left to right respectively. Image 1 is the uncorrected in-focus lateral plane, image 3 is the uncorrected though-focus image. Images 2 and 4 are the images corrected with adaptive optics using mirao. Images are enlarged to show detail and compressed at 85% quality JPEG. |
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| OCT |
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Human retina in vivo - Images courtesy of Pr. Wolgang Drexler, Cardiff University
The images below of human photoreceptor cells were acquired using mirao coupled with SD-OCT. |
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For more information, please call +33 (0)1 64 86 15 60 or send us a mail by clicking here.
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